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Lung-on-Chip

The Dynamic42 Lung-on-Chip can be used to evaluate uptake and mechanistical features of pre-clinical drug candidates and chemicals that follow a respiratory delivery via the lung epithelium. It further qualifies to investigate air-borne infectious diseases.

We use our perfusable Dynamic42 biochip platform to provide an innovative concept to setup in vivo-like microenvironments of the human lung by reconstructing perfusable vascular tissue in combination with lung epithelium exposed to air.

Specifications

The Dynamic42 Lung-on-Chip comprises two compartments: a vascular compartment and an alveolar compartment. The vasculature is formed by an endothelial lining. The alveolar compartment comprises lung epithelial cells and tissue-resident alveolar macrophages. The alveolar tissue is exposed to air and nourished from the vasculature below (air-liquid-interface culture).

The Dynamic42 Lung-on-Chip can be operated with various cell sources. Please get in contact for further details.

macrophage-2

Macrophage

epithelial-cell-2

Epithelial Cell

endothelial-cell-2

Endothelial Cell

Two models can be operated in parallel on one Dynamic42 biochip.

The model has been tested in the following Dynamic42 biochips: BC001 and BC002.

Characteristics

Enhanced marker expression

Intense cell-cell communication

Physiologic biomechanical stimulation via flow

Secretory function

Immunocompetence

The Dynamic42 Lung-on-Chip can be operated up to 14 days with high vitality, stable barrier function and stable marker expression.

Slide 1

D42 Lung-on-Chip: Endothelial cells comprise the vasculature of our Lung-on-Chip model. Endothelial cells express von Willebrand factor (vWf) stained in green and CD31 stained in orange. vWf plays a major role in platelet adhesion and in protective complex formation with factor VIII. CD31 mediates mechano-sensing of biomechanical stimuli and leukocyte adhesion to the vessel wall.

Slide 1

D42 Lung-on-Chip: Alveolar epithelial cells comprise the epithelial lining in our Alveolus-on-Chip model variant. Epithelial cells show strong and continuous E-cadherin staining in orange. E-cadherin is a major component of alveolar adherens junctions.

Slide 1

D42 Lung-on-Chip: Alveolar epithelial cells comprise the epithelial lining in our Alveolus-on-Chip model variant. Epithelial cells show strong and continuous E-cadherin staining in orange and surfactant protein A (SP-A) in red. SP-A is secreted by alveolar epithelial cells and important for alveolar clearance function. It opsonizes microbial particles to facilitate their phagocytosis by macrophages.

Slide 1

D42 Lung-on-Chip: Bronchial epithelial cells comprise the epithelial lining in our Bronchius-on-Chip model variant. Epithelial cells show strong and continuous expression of Zona Occludens 1 (ZO-1) in green. ZO-1 interacts with occluding and claudins to support the epithelial barrier.

Slide 1

D42 Lung-on-Chip: Bronchial epithelial cells comprise the epithelial lining in our Bronchius-on-Chip model variant. E-cadherin is stained in orange. E-cadherin is a major component of bronchial adherens junctions. The picture shows an overlay with ZO-1 stained in green.

Slide 1

D42 Lung-on-Chip: Bronchial epithelial cells comprise the epithelial lining in our Bronchius-on-Chip model variant. After 4 weeks of differentiation under air-liquid-interface (ALI) epithelial cells express acetylated alpha tubulin (acαTub) stained in orange and mucin 5AC (Muc5AC) stained in green. acαTub is present in stable microtubular structures such as cilia on top of the cells. Muc5AC is secreted, binds pathogens and is involved in muco-ciliar clearance.

Slide 1

D42 Lung-on-Chip: Bronchial epithelial cells comprise the epithelial lining in our Bronchius-on-Chip model variant. After 4 weeks of differentiation under air-liquid-interface (ALI) epithelial cells express acαTub stained in orange, Muc5AC stained in green and uteroblobin/ clara cell 10 kDa protein (CC10) stained in red. CC10 is produced by mucosal cells in the lung such as non-ciliated bronchiolar club cells. Its precise physiological role is still of debate, but may involve immunomodulation.

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Applications

  • Toxicity profiling
  • Uptake / Transport studies
  • Inflammatory Diseases
  • Infectious Diseases

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